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gear GEAR questions please
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gear GEAR questions please - 10-08-2006, 01:41 AM

Okay I have a few questions regarding transferring and filtering gear, etc. Hope I can get some answers.




~Say i have some oil in sachets, and I want to filter them into vials. Now i use a .22 whatman but what is the procedure on this? Do I just draw with the needle, place filter on vial and shoot needle through filter into the sterile vial?


~When buying sterile sealed vials does that mean that they are already crimped with a stopper or just sealed in airtight wrapper/container?
If they do not have stoppers, and I use butyl stoppers do they just plug into the vial or would i still need to get a crimper and crimp them?


~Lastly, I know this has been covered but I hope this isnt too much of a burden to answer but what type of sterile oil is best? Are they pretty much all the same or would something like sesame oil have plusses to fat burning etc. I mean what is the choice out of grapeseed, cottonseed, sesame, and walnut oils?

Im getting ready to order all my gear gear so I want to make sure I get it right the first time. I apprieciate the help in advance.
   
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10-08-2006, 08:45 AM

question 1 - yup,. A vent pin is needed or draw a vaccuum in your sterile vial,which is how I do it.

Question 2- Yes buy sealed and pre-crimped vials

Question 3- Grapeseed seems to be the healthiest and is very thin but sesame is thinner. I use grape seed for everything
   
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10-08-2006, 10:05 AM

The whatman filter will screw on your syringe... then your needle will screw on the whatman filter.

Like BoomBaby said youll want to put a needle tip only into the sealed Vial to
releive the pressure...Then use your whatman,needle combo to filter it Into the SEALED , sterile Vial.
youll see once you have it its a pretty simple process.
Any Usp Sterile oil you can get from a research company or kit supplier will work. I always reccomend to guys to use
www.Kaotikchemistry.com there good honest people and will also help with any questions you may have.


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Thanks alot
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Thanks alot - 10-08-2006, 11:33 AM

thanks alot fellahs. You responses have been of great help. I was especially confused on how the whatman works but Rajjin cleared that up for me nicely. As far as the oils i was going to go with grapeseed from the start but i thought sesame might have anti fat properties, but ill go with grapeseed because of what was stated here and i also heard it has antioxidant properties.
   
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10-08-2006, 03:05 PM

one other thing to note which is very important: use extreme patience when pushing the oil through the filter. it is a very slow process and you cannot try to push the oil through to fast or you risk a blowout of the filter which would cause you to have to refilter everything all over again. i did this once and it sucked but i learned my lesson. it should take ya a couple minutes to fliter a 10ml syringe filled with the solution.
i use grapeseed oil also. i buy it from the grocery store then heat it to 250degree's in the oven for 45 minutes then run it through a filter before i get ready to use it. easier way is to just buy from a research supply company like the one rajjin recommends. they are very good with a great reputation
   
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