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Go Back   Anabolic Steroids Discussion and Bodybuilding Forum > Anabolic Science Section > Anabolic Steroid & Serum Testing

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Extreme Peptide IGF-LR3
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Extreme Peptide IGF-LR3 - 02-18-2013, 12:49 PM

I have have said over and over again that my reputation and dignity means more than anything. There is nothing a peptide company can offer me to change my views on the truth. I told every sponsor that i will post exactly what is given to me by the lab and that there will be no sugar coating at all.

The testing was done by Texas A&M protein and Chemistry lab and they are one of the premier labs for testing peptides in this country. I suggest you guys read the thread "Demand more from your sponsors" to understand the testing methods used.


Extreme Peptide LR3


To explain how to read each i am going to get a little nerdy. IGF LR3 has a mass weight of 9111 daltons. Assuming we could get a 100% pure peptide that was exactly we should get a very tall and narrow peak right at 9111 area. You can see from the MALDI-MASS that we have a very nice peak at the 9111 area but there is also another peak at the 9400 area. This is approximately a 3 amino acid difference in weight. You can see two smaller peaks in the 4000 range but small enough that really not a big deal. The sample is obviously not 100% pure LR3. The good new is that we do have a lot of protein at the 9111 area. So that is the MS.

Now the XL worksheet you see is the AAA (amino acid analysis) this is where the sample is actually broken down and we can see how many of each amino acid is present in a single chain. For example how many glutamine, how many leucine or valine etc. Since we know how many of each are SUPPOSED TO be in a real LR3 we can compare the PERCENTAGE of each known amino acid to be there vs what we actually get from sample tested. This is where it really things matter. That is because i can make a peptide long enough that weighs close to 9111 daltons and get a mass spec graph but it can be pure crap and have nothing to even do with IGF LR3. Hope this makes sense.

We can see from the XL worksheet that the sample was ran in a tripplicate assay for even better accuracy and the number of nanomoles of each amino acid in the chain.

Column C shows number of each amino acid that is supposed to be in a real LR3
Column D shows % of weight of each amino acid that is supposed to be in a real LR3
Column E shows % of weight of each amino acid that we actually got from sample being tested in this case Extreme's LR3

You can see that predicted vs what we got is very close and told by lab director completely within range of lab error and they include a 1.5% up or down either direction completely acceptable. So we can see that the Extremes sample had the right amount of each amino acid in the chain.

If you look at C:43 you can see that there was 989 micrograms of protein in total in the vial which means 0.98 mg which is 98% of 1mg. There was some Histidine amino acids in a very small amount found which LR3 should have no Histidine and lab director explained that this is most likely Histidine that is left over from the tagging process that may have not been cleaved off but extremely small which also is the cause of the 2nd spike we see on the MS graph at 9400 area.

Overall he felt very comfortable to say this is LR3 Enjoy




My thoughts:


First i am pretty impressed that we even had any damn LR3 in any of these vials cause i was very skeptical to begin with despite the source. I do not think any of them are "bad." I think it is matter of good or better. I have already contacted OstaPresident and sent him the raw data and have even offered to setup so he can send a vial directly to lab for analysis. I dont want anyone to think there are any fishy stuff going on.

SRC by pure analysis looks very good however the fact that they are the most expensive out of the bunch and had only 0.8mg which is about 20% short of advertised makes them great but very pricey. You pretty much have to add 20% to their price to get a real price on what 1.0mg would cost.

I think Extreme, Maxim and Purchase Peptide have a great product. We know for sure we have LR3 and pretty much as close as to 1.0mg as you can get. The histidine is very small when you look at % of amino acid involved. The lab tells me less then 5-8% may be His-tagged. So even if you subtract 8% from 0.95mg you still end up with more LR3 that is untagged and probably at a better price. It was revealed and confirmed by Extreme that Ergo's USA LR3 is the same as Extreme and they buy from exact same source so i did not want to spend another $400 to test the exact same product again since Extreme's tested so good.

Osta unfortunately had the peak at 7000 which neither lab or i can explain and had only 0.73mg of total protein in the vial. I am hoping they discuss this with their manufacturer and come back with a better batch to test or we can re-do the test if they would like.

Please do not turn this into mud slinging etc. The data is clear and evident. If you have direct questions about interpretations of the data etc please feel free to ask and i will respond.

At this point my job with LR3 is done. I think there are a lot of great options out there. I would like to turn my attention to DES. In my humble opinion, DES is even a better compound than LR3 but much more difficult to produce. We are completely out of funds for Des testing. If members would like to donate it would be great so we can do similar testing for DES. These tests normally cost $1000-1500 per sample but due to my pharma connections, the lab is doing it at cost which comes to ~400 when counting shipping. If members would like to donate please message me so i can send you paypal address so we can carry same testing with DES.
   
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02-18-2013, 01:01 PM

Great post!! Thank you





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02-18-2013, 01:27 PM

Thanks for all the in depth info.
   
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02-18-2013, 03:43 PM

This is great info! Helps to keep the sponsors quality control in check.

I only wish the same could be done for gear too. Would be great to be able to see what they are offering in the way of quality.

Can't wait to see more of these done!
   
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02-18-2013, 07:58 PM

What a contribution! Thanks.
   
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02-18-2013, 08:31 PM

Great review and very thorough in truth. Greatly appreciate this and will continue doing business with a great sponsor..
   
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02-18-2013, 08:36 PM

Hey alpha, didnt you test ergo's as well i thought? or is that the des thats up and coming?


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02-18-2013, 09:55 PM

Thanks for all your efforts


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02-19-2013, 08:31 AM

Awesome post! Very helpful and educational! thank you!




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02-19-2013, 08:53 AM

Quote:
Originally Posted by omegachewy View Post
Hey alpha, didnt you test ergo's as well i thought? or is that the des thats up and coming?
Extreme and ergo use the same supplier, so testing would have been a waste of money.
   
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Extreme Peptide IGF-LR3
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Extreme Peptide IGF-LR3 - 02-20-2013, 01:38 PM

Great info here and definitely makes me feel comfortable in purchasing lr3 again cause like you Alpha I was skeptical.
   
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02-20-2013, 05:45 PM

Quote:
Originally Posted by Magnus82 View Post
Extreme and ergo use the same supplier, so testing would have been a waste of money.
ah thank you.


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02-20-2013, 06:20 PM

Me and good igf1r3 are brothers
   
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