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Lifespan of IGF-1
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PRIDE
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Lifespan of IGF-1 - 01-08-2013, 01:18 AM

The stability of a liquid solution of LR3IGF-I was monitored for a
period of two years at storage conditions of -20 C, +4 C, +22 C,
and +37 C. The final concentration of LR3IGF-I was in acetic acid.
At various time points, samples were taken and compared to a
lyophilized control (stored at 4 C). Listed below are the stability
results for each respective storage condition.

Storage Condition: -20 C (-4 F)
Biological Potency No Change up to 2 years
Immunological Activity No Change up to 2 years
Mobility of Protein No Change up to 2 years
Elution Profile by reversed phased HPLC No Change up to 2 years

Storage Condition: +4 C (39.2 F)
Biological Potency No Change up to 2 years
Immunological Activity No Change up to 2 years
Mobility of Protein No Change up to 2 years
Elution Profile by reversed phased HPLC No Change up to 2 years

Storage Condition: +22 C (71.6 F)
Biological Potency No Change up to 2 years
Immunological Activity No Change up to 2 years
Mobility of Protein No Change up to 2 years
Elution Profile by reversed phased HPLC No Change up to 2 years

Storage Condition: +37 C (98.6 F)
Biological Potency No Change up to 1 year
Immunological Activity No Change up to 1 year
Mobility of Protein No Change up to 1 year
Elution Profile by reversed phased HPLC No Change up to 1 year

Bacteriostatic can still be used, but IGF calls for Acetic Acid since it`s the PH levels in the acid that maintain the integrity and quality of the IGF...BW reconstitution will last for about 1 to 2 weeks on average...


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Measuring IGF: Step by step process
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PRIDE
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Measuring IGF: Step by step process - 01-08-2013, 01:19 AM

first remove the vacuum from the vial, by injecting air into it. when the vacuum is gone it will no longer pull the plunger down

you can use as much AA as you like. the original kits came with 1ml. most time when i drew it into a syringe, it was't even that much, which of course is a problem. chemone includes a little more than 2ml so if you want to use 1ml, fine, 2ml, fine. there will be enough.

i personally use 2ml because it is easier to measure later when you draw it into a syringe.

after removing the vacuum SLOWLY add AA to the vial. try not to let the stream of aa hit the powder directly, try to let it run down the glass into the powder. when the aa is in gently swirl the vial until all is disolved. do not shake.

a 1ml syringe is marked like this llll10llll20llll30llll40llll50llll60...etc
a 1/2 ml syringe is like this llll5llll10llll15llll20llll25llll30

on a 1ml each line represents 2 units 2,4,6,8,10
on a 1/2 ml each line is 1 unit 1,2,3,4,5,6,7,8,9,10. this makes it easier to measure.

so if you use 1ml of aa, on a 1ml pin, each line is 20mcg. on a 1/2ml pin each line is 10mcg

if you use 2ml of aa, on a 1ml pin each line is 10mcg, on a 1/2 ml each line is 5mcg...the 5line is 25mcg, 10 line is 50mcg...etc

so you see that the easiest way to measure is using 2ml and a 1/2ml pin

your igf is stored in aa solution, nacl is added to dilute at the time of injection. draw twice as much nacl as igf. so if you draw igf to the ten line, draw nacl to the 30 line and you are ready to go.


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