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Considering brewing for first time

joshck77

Registered User
Feb 7, 2014
58
0
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Water-Based Test Suspension2g Test Base~14.25ml Bac Water
.6ml BA
.4ml Polysorbate 80
4ml BB
1. Add Bacteriostatic water to a sterile 20ml vial.2. Add test base, BA, ps80, and 2ml BB to a beaker. Heat until clear (note - test base has a very high melting point so if it smokes a bit its OK)3. Let test base cool a bit4. Draw up solution in a 5cc syringe. Attach a .45 syringe filter to the 5cc syringe and filter into the Bac water in the vial. Do this quickly so the test base doesn't have time to cool in the water. The test base will titrate out of the solution. IMPORTANT - Only filter 1cc at a time as to not let the base crash in the syringe.... VERY IMPORTANT!!5. Swirl gently until mixed and then shake the shit out of it.6. Purge the filter with 2ml of BB, shake and swirl.This will yield 20ml of micronized water based test suspension at 100mg/ml