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sterlization

Bigmills

Registered User
Jun 27, 2016
42
0
0
How high can you go on the vacuum pump with the media bottle?
As long as your media bottle is glass around 12 PSI is perfect. you could probably go up to 13 or 14,but anything above that you are risking blowing out the filter. If you have a plastic media bottle I wouldn't suggest using a pump at all..

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1bigdog

Banned
May 26, 2016
117
0
0
I use glass and can handle 24psi. Its wheaton which has never even tested their glass for pressure so youd be surprised.
 

khungus03

New member
Jun 2, 2013
23
0
1
Autoclave is best, pressure cooker is essentially an autoclave so that'd be second. Dry heat would be third and will work for glassware but I wouldn't do the cap this way. You'll want to bake the media bottle at 400° for 60 minutes, do this with aluminum foil covering the open top so when it cools the inside of the bottle is not exposed to the environment.